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Modulating hemoglobin nitrite reductase activity through allostery: A mathematical model

Rong, Z and Alayash, AI and Wilson, MT and Cooper, CE (2013) 'Modulating hemoglobin nitrite reductase activity through allostery: A mathematical model.' Nitric Oxide - Biology and Chemistry, 35. 193 - 198. ISSN 1089-8603

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Abstract

The production of nitric oxide by hemoglobin (Hb) has been proposed to play a major role in the control of blood flow. Because of the allosteric nature of hemoglobin, the nitrite reductase activity is a complex function of oxygen partial pressure PO2. We have previous developed a model to obtain the micro rate constants for nitrite reduction by R state (kR) and T state (kT) hemoglobin in terms of the experimental maximal macro rate constant kNmaxand the corresponding oxygen concentration PO2max. However, because of the intrinsic difficulty in obtaining accurate macro rate constant kN, from available experiments, we have developed an alternative method to determine the micro reaction rate constants (kRand kT) by fitting the simulated macro reaction rate curve (kNversus PO2) to the experimental data. We then use our model to analyze the effect of pH (Bohr Effect) and blood ageing on the nitrite reductase activity, showing that the fall of bisphosphoglycerate (BPG) during red cell storage leads to increase NO production. Our model can have useful predictive and explanatory power. For example, the previously described enhanced nitrite reductase activity of ovine fetal Hb, in comparison to the adult protein, may be understood in terms of a weaker interaction with BPG and an increase in the value of kTfrom 0.0087 M-1s-1to 0.083 M-1s-1. © 2013 Elsevier Inc. All rights reserved.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Users 161 not found.
Date Deposited: 20 Dec 2014 22:06
Last Modified: 26 Jun 2018 19:15
URI: http://repository.essex.ac.uk/id/eprint/10190

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