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Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods.

Hui, Jerome HL and Marco, Antonio and Hunt, Suzanne and Melling, Janet and Griffiths-Jones, Sam and Ronshaugen, Matthew (2013) 'Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods.' Nucleic Acids Res, 41 (5). 3352 - 3361. ISSN 1362-4962

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In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites.

Item Type: Article
Uncontrolled Keywords: Animals, Cell Line, Chromosome Mapping, Drosophila melanogaster, Evolution, Molecular, Gene Expression Regulation, Developmental, Genes, Homeobox, Genes, Insect, Genetic Loci, MicroRNAs, Phylogeny, RNA Interference, Transcription, Genetic, Tribolium
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Antonio Marco
Date Deposited: 23 Dec 2014 15:56
Last Modified: 30 Sep 2019 23:15

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