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Gβ phosphorylation is critical for efficient chemotropism in yeast

DeFlorio, Reagan and Brett, Marie-Elena and Waszczak, Nicholas and Apollinari, Elisabetta and Metodiev, Metodi V and Dubrovskyi, Oleksii and Eddington, David and Arkowitz, Robert A and Stone, David E (2013) 'Gβ phosphorylation is critical for efficient chemotropism in yeast.' Journal of Cell Science, 126 (14). pp. 2997-3009. ISSN 0021-9533

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<jats:p>Mating yeast cells interpret complex pheromone gradients and polarize their growth in the direction of the closest partner. Chemotropic growth depends on both the pheromone receptor and its associated G-protein. Upon activation by the receptor, Gα dissociates from Gβγ and Gβ is subsequently phosphorylated. Free Gβγ signals to the nucleus via a MAPK cascade and recruits Far1-Cdc24 to the incipient growth site. It is not clear how the cell establishes and stabilizes the axis of polarity, but this process is thought to require local signal amplification via the Gβγ-Far1-Cdc24 chemotropic complex, as well as communication between this complex and the activated receptor. Here we show that a mutant form of Gβ that cannot be phosphorylated confers defects in directional sensing and chemotropic growth. Our data suggest that phosphorylation of Gβ plays a role in localized signal amplification and in the dynamic communication between the receptor and the chemotropic complex, which underlie growth site selection and maintenance.</jats:p>

Item Type: Article
Uncontrolled Keywords: G beta phosphorylation; Saccharomyces cerevisiae; Chemotropism; Polarized growth; Reorientation; Yeast mating response
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 23 Dec 2014 16:04
Last Modified: 18 Aug 2022 12:40

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