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Phosphorylation of gb is crucial for efficient chemotropism in yeast

DeFlorio, R and Brett, ME and Waszczak, N and Apollinari, E and Metodiev, MV and Dubrovskyi, O and Eddington, D and Arkowitz, RA and Stone, DE (2013) 'Phosphorylation of gb is crucial for efficient chemotropism in yeast.' Journal of Cell Science, 126 (14). 2997 - 3009. ISSN 0021-9533

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Abstract

Mating yeast cells interpret complex pheromone gradients and polarize their growth in the direction of the closest partner. Chemotropic growth depends on both the pheromone receptor and its associated G-protein. Upon activation by the receptor, Gα dissociates from Gβγ and Gβ is subsequently phosphorylated. Free Gβγ signals to the nucleus via a MAPK cascade and recruits Far1-Cdc24 to the incipient growth site. It is not clear how the cell establishes and stabilizes the axis of polarity, but this process is thought to require local signal amplification via the Gβγ-Far1-Cdc24 chemotropic complex, as well as communication between this complex and the activated receptor. Here we show that a mutant form of Gβ that cannot be phosphorylated confers defects in directional sensing and chemotropic growth. Our data suggest that phosphorylation of Gβ plays a role in localized signal amplification and in the dynamic communication between the receptor and the chemotropic complex, which underlie growth site selection and maintenance. © 2013. Published by The Company of Biologists Ltd.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Users 161 not found.
Date Deposited: 23 Dec 2014 16:04
Last Modified: 17 Aug 2017 17:48
URI: http://repository.essex.ac.uk/id/eprint/10360

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