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Amino acid conservation and interactions in rhodopsin: Probing receptor activation by NMR spectroscopy

Pope, A and Eilers, M and Reeves, PJ and Smith, SO (2014) 'Amino acid conservation and interactions in rhodopsin: Probing receptor activation by NMR spectroscopy.' Biochimica et Biophysica Acta - Bioenergetics, 1837 (5). 683 - 693. ISSN 0005-2728

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Abstract

Rhodopsin is a classical two-state G protein-coupled receptor (GPCR). In the dark, its 11-cis retinal chromophore serves as an inverse agonist to lock the receptor in an inactive state. Retinal-protein and protein-protein interactions have evolved to reduce the basal activity of the receptor in order to achieve low dark noise in the visual system. In contrast, absorption of light triggers rapid isomerization of the retinal, which drives the conversion of the receptor to a fully active conformation. Several specific protein-protein interactions have evolved that maintain the lifetime of the active state in order to increase the sensitivity of this receptor for dim-light vision in vertebrates. In this article, we review the molecular interactions that stabilize rhodopsin in the dark-state and describe the use of solid-state NMR spectroscopy for probing the structural changes that occur upon light-activation. Amino acid conservation provides a guide for those interactions that are common in the class A GPCRs as well as those that are unique to the visual system. This article is part of a Special Issue entitled: Retinal Proteins - You can teach an old dog new tricks. © 2013 Elsevier B.V.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Users 161 not found.
Date Deposited: 26 Sep 2014 10:29
Last Modified: 20 Sep 2019 21:15
URI: http://repository.essex.ac.uk/id/eprint/10405

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