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Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

Worrall, Jonathan AR and Górna, Maria and Crump, Nicholas T and Phillips, Lara G and Tuck, Alex C and Price, Amanda J and Bavro, Vassiliy N and Luisi, Ben F (2008) 'Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.' Journal of Molecular Biology, 382 (4). 870 - 883. ISSN 0022-2836

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The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo.

Item Type: Article
Uncontrolled Keywords: Escherichia coli, Multienzyme Complexes, Endoribonucleases, Phosphopyruvate Hydratase, Polyribonucleotide Nucleotidyltransferase, RNA Helicases, Escherichia coli Proteins, Host Factor 1 Protein, Recombinant Proteins, RNA Precursors, RNA, Bacterial, Nucleic Acid Conformation, Genetic Vectors, DEAD-box RNA Helicases
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 11 Oct 2011 11:22
Last Modified: 28 Jun 2021 13:15

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