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Reconstitution and Analysis of the Multienzyme Escherichia coli RNA Degradosome

Worrall, JAR and Górna, M and Crump, NT and Phillips, LG and Tuck, AC and Price, AJ and Bavro, VN and Luisi, BF (2008) 'Reconstitution and Analysis of the Multienzyme Escherichia coli RNA Degradosome.' Journal of Molecular Biology, 382 (4). 870 - 883. ISSN 0022-2836

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Abstract

The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo. © 2008 Elsevier Ltd. All rights reserved.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 11 Oct 2011 11:22
Last Modified: 14 Oct 2019 17:18
URI: http://repository.essex.ac.uk/id/eprint/1078

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