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Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

Worrall, Jonathan AR and Górna, Maria and Crump, Nicholas T and Phillips, Lara G and Tuck, Alex C and Price, Amanda J and Bavro, Vassiliy N and Luisi, Ben F (2008) 'Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.' Journal of Molecular Biology, 382 (4). pp. 870-883. ISSN 0022-2836

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Abstract

The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo.

Item Type: Article
Uncontrolled Keywords: Escherichia coli; Multienzyme Complexes; Endoribonucleases; Phosphopyruvate Hydratase; Polyribonucleotide Nucleotidyltransferase; RNA Helicases; Escherichia coli Proteins; Host Factor 1 Protein; Recombinant Proteins; RNA Precursors; RNA, Bacterial; Nucleic Acid Conformation; Genetic Vectors; DEAD-box RNA Helicases
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 11 Oct 2011 11:22
Last Modified: 18 Aug 2022 11:32
URI: http://repository.essex.ac.uk/id/eprint/1078

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