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Generalist hydrocarbon-degrading bacterial communities in the oil-polluted water column of the North Sea

Chronopoulou, Panagiota-Myrsini and Sanni, Gbemisola O and Silas-Olu, Daniel I and van der Meer, Jan Roelof and Timmis, Kenneth N and Brussaard, Corina PD and McGenity, Terry J (2015) 'Generalist hydrocarbon-degrading bacterial communities in the oil-polluted water column of the North Sea.' Microbial Biotechnology, 8 (3). pp. 434-447. ISSN 1751-7915

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The aim of this work was to determine the effect of light crude oil on bacterial communities during an experimental oil spill in the North Sea and in mesocosms (simulating a heavy, enclosed oil spill), and to isolate and characterize hydrocarbon-degrading bacteria from the water column. No oil-induced changes in bacterial community (3m below the sea surface) were observed 32h after the experimental spill at sea. In contrast, there was a decrease in the dominant SAR11 phylotype and an increase in Pseudoalteromonas spp. in the oiled mesocosms (investigated by 16S rRNA gene analysis using denaturing gradient gel electrophoresis), as a consequence of the longer incubation, closer proximity of the samples to oil, and the lack of replenishment with seawater. A total of 216 strains were isolated from hydrocarbon enrichment cultures, predominantly belonging to the genus Pseudoaltero monas; most strains grew on PAHs, branched and straight-chain alkanes, as well as many other carbon sources. No obligate hydrocarbonoclastic bacteria were isolated or detected, highlighting the potential importance of cosmopolitan marine generalists like Pseudoalteromonas spp. in degrading hydrocarbons in the water column beneath an oil slick, and revealing the susceptibility to oil pollution of SAR11, the most abundant bacterial clade in the surface ocean.

Item Type: Article
Uncontrolled Keywords: Bacteria; Pseudoalteromonas; Water; Hydrocarbons; Oils; DNA, Bacterial; DNA, Ribosomal; RNA, Ribosomal, 16S; Water Pollutants; Cluster Analysis; Sequence Analysis, DNA; Seawater; Phylogeny; Biotransformation; Molecular Sequence Data; North Sea; Chrysophyta; Biota
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 15 Oct 2014 10:43
Last Modified: 18 Aug 2022 11:09

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