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Construction and characterisation of a gridded chicken cosmid library with four-fold genomic coverage

Buitkamp, J and Ewald, D and Schalkwyk, L and Weiher, M and Masabanda, J and Sazanov, A and Lehrach, H and Fries, R (1998) 'Construction and characterisation of a gridded chicken cosmid library with four-fold genomic coverage.' Animal Genetics, 29 (4). pp. 295-301. ISSN 0268-9146

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Gridded genomic libraries are crucial for the positional candidate gene approach. For this purpose we constructed a gridded genomic library from a female chicken using the vector sCos 1. About 110 000 cosmid clones were grown and replicated in 384-well plates. An average insert size of 39 kb was calculated from the analysis of 68 randomly selected clones. No chimerism could be observed from 31 in situ hybridisations. One replica of the library (number 125) has been transferred to the Resource Centre/Primary Database (RZPD) of the German Human Genome Project (DHGP). The whole library was gridded onto four nylon filters at high density for efficient identification of cosmid clones by colony hybridisation. Twenty-two probes were used for screening the library and each of them gave at least one positive signal. This result is in good agreement with a four-fold coverage of the genome as estimated from the insert length and number of recombinant clones. This library provides a powerful tool for rapid physical mapping and complex analysis of the chicken genome.

Item Type: Article
Uncontrolled Keywords: avidin gene cluster; chicken; cosmid cloning; fluorescence in situ hybridisation; gridded cosmid library
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Jul 2017 13:32
Last Modified: 18 Aug 2022 11:41

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