Research Repository

Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2

Charlebois, RL and Schalkwyk, LC and Hofman, JD and Doolittle, WF (1991) 'Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2.' Journal of Molecular Biology, 222 (3). 509 - 524. ISSN 0022-2836

Full text not available from this repository.

Abstract

An integrated approach of "bottom up" and "top down" mapping has produced a minimal set of overlapping cosmid clones covering 96% of the 4140 kilobase-pairs (kbp) Haloferax volcanii DS2 genome and a completely closed physical map. This genome is partitioned into five replicons: a 2920 kbp chromosome and four plasmids, of 690 kbp (pHV4), 442 kbp (pHV3), 86 kbp(pHV1) and 6·4 kbp (pHV2). A restriction map for six infrequently-cutting restriction enzymes was constructed, representing a total of 903 sites in the cloned DNA. We have placed the two ribosomal RNA operons, the genes for 7 S RNA and for RNaseP RNA and 22 protein-coding genes on the map. Restriction site frequencies show significant variation in different portions of the genome. The regions of high site density correspond to halobacterial satellite or FII DNA which includes two small regions of the chromosome, the plasmids pHV1 and pHV2, a nd half of pHV4, but not pHV3. © 1991.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 03 Aug 2017 11:28
Last Modified: 12 Jun 2018 10:15
URI: http://repository.essex.ac.uk/id/eprint/11001

Actions (login required)

View Item View Item