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High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe

Hoheisel, J (1993) 'High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe.' Cell, 73 (1). pp. 109-120. ISSN 0092-8674

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Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, were ordered by hybridlzing genetic markers and individual clones from the two libraries. Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. Restriction digests were generated as an independent confirmation of the clone order. The high resolution clone map was aligned to the genetic map and the physical Notl and YAC maps. The usefulness of the various mapping techniques and cloning procedures could be assessed upon the different data sets. © 1993.

Item Type: Article
Uncontrolled Keywords: Chromosomes, Fungal; Bacteriophage P1; Schizosaccharomyces; DNA Probes; Chromosome Mapping; Nucleic Acid Hybridization; Base Sequence; Repetitive Sequences, Nucleic Acid; Genomic Library; Cosmids; Molecular Sequence Data
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 02 Aug 2017 13:18
Last Modified: 15 Jan 2022 00:49

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