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High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe

Hoheisel, JD and Maier, E and Mott, R and McCarthy, L and Grigoriev, AV and Schalkwyk, LC and Nizetic, D and Francis, F and Lehrach, H (1993) 'High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe.' Cell, 73 (1). 109 - 120. ISSN 0092-8674

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Abstract

Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h - , were ordered by hybridlzing genetic markers and individual clones from the two libraries. Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. Restriction digests were generated as an independent confirmation of the clone order. The high resolution clone map was aligned to the genetic map and the physical Notl and YAC maps. The usefulness of the various mapping techniques and cloning procedures could be assessed upon the different data sets. © 1993.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 02 Aug 2017 13:18
Last Modified: 17 Aug 2017 17:46
URI: http://repository.essex.ac.uk/id/eprint/11036

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