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Genetic analysis of the mouse brain proteome

Klose, Joachim and Nock, Christina and Herrmann, Marion and Stühler, Kai and Marcus, Katrin and Blüggel, Martin and Krause, Eberhard and Schalkwyk, Leonard C and Rastan, Sohaila and Brown, Steve DM and Büssow, Konrad and Himmelbauer, Heinz and Lehrach, Hans (2002) 'Genetic analysis of the mouse brain proteome.' Nature Genetics, 30 (4). pp. 385-393. ISSN 1061-4036

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Abstract

Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8, 767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected 1, 324 polymorphic proteins from the European collaborative interspecific backcross. Of these, we mapped 665 proteins genetically and identified 466 proteins by mass spectrometry. Qualitatively polymorphic proteins, to 96%, reflect changes in conformation and/or mass. Quantitatively polymorphic proteins show a high frequency (73%) of allele-specific transmission in codominant heterozygotes. Variations in protein isoforms and protein quantity often mapped to chromosomal positions different from that of the structural gene, indicating that single proteins may act as polygenic traits. Genetic analysis of proteomes may detect the types of polymorphism that are most relevant in disease-association studies.

Item Type: Article
Uncontrolled Keywords: Brain; Animals; Mice; Protein Isoforms; DNA; Genetic Markers; Electrophoresis, Gel, Two-Dimensional; Chromosome Mapping; Cloning, Molecular; Crosses, Genetic; Polymerase Chain Reaction; Nucleic Acid Hybridization; Protein Conformation; Genotype; Heterozygote; Phenotype; Polymorphism, Genetic; Alleles; Models, Genetic; Female; Mass Spectrometry; Genetic Linkage
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 02 Aug 2017 16:25
Last Modified: 15 Jan 2022 00:49
URI: http://repository.essex.ac.uk/id/eprint/11047

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