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ETn insertion in the mouse Adcy1 gene: transcriptional and phylogenetic analyses

Leong, Wey L and Dobson, Melanie J and Logsdon, Jr., John M and Abdel-Majid, Raja M and Schalkwyk, Leonard C and Guernsey, Duane L and Neumann, Paul E (2000) 'ETn insertion in the mouse Adcy1 gene: transcriptional and phylogenetic analyses.' Mammalian Genome, 11 (2). pp. 97-103. ISSN 0938-8990

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Early retrotransposons (ETn) are murine transposable elements, bearing some structural similarity to integrated proviruses, and can be insertional mutagens. We have recently identified the causative mutation of the barrelless (Adcy1(brl)) phenotype as an integration of a 5.7-kb ETn in an intron of the adenylyl cyclase type I (Adcy1) gene. In the present study, Northern blot analysis shows that the ETn insertion results in loss of the normal Adcy1 transcript, a finding consistent with the loss-of-function Adcy1(brl) mutation, and generation of shorter transcripts. These aberrant transcripts are the products of abnormal RNA splicing and termination owing to the inserted sequence, and transcription initiation within the 3' long terminal repeat (LTR) of the ETn. The DNA sequences of the LTRs were compared in phylogenetic analyses with LTRs from 22 other ETn-related sequences. Three distinct families of ETn sequences can be identified on the basis of their LTRs. The ETn found in Adcy1(brl) is a member of a family that includes all classified ETn elements known to have recently transposed. Further, of the four known solitary (solo) LTRs, we have identified two that show evidence of recombination between LTRs from different ETn families.

Item Type: Article
Uncontrolled Keywords: Brain; Animals; Mice, Inbred C57BL; Mice; Mice, Mutant Strains; Retroelements; RNA, Messenger; Blotting, Northern; Mutagenesis, Insertional; Phylogeny; Base Sequence; Sequence Homology, Nucleic Acid; Molecular Sequence Data; Adenylyl Cyclases
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 02 Aug 2017 11:35
Last Modified: 15 Jan 2022 00:49

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