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Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome.

McCarthy, L and Hunter, K and Schalkwyk, L and Riba, L and Anson, S and Mott, R and Newell, W and Bruley, C and Bar, I and Ramu, E (1995) 'Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome.' Proceedings of the National Academy of Sciences, 92 (12). pp. 5302-5306. ISSN 0027-8424

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Abstract

<jats:p>The ability to carry out high-resolution genetic mapping at high throughput in the mouse is a critical rate-limiting step in the generation of genetically anchored contigs in physical mapping projects and the mapping of genetic loci for complex traits. To address this need, we have developed an efficient, high-resolution, large-scale genome mapping system. This system is based on the identification of polymorphic DNA sites between mouse strains by using interspersed repetitive sequence (IRS) PCR. Individual cloned IRS PCR products are hybridized to a DNA array of IRS PCR products derived from the DNA of individual mice segregating DNA sequences from the two parent strains. Since gel electrophoresis is not required, large numbers of samples can be genotyped in parallel. By using this approach, we have mapped &gt; 450 polymorphic probes with filters containing the DNA of up to 517 backcross mice, potentially allowing resolution of 0.14 centimorgan. This approach also carries the potential for a high degree of efficiency in the integration of physical and genetic maps, since pooled DNAs representing libraries of yeast artificial chromosomes or other physical representations of the mouse genome can be addressed by hybridization of filter representations of the IRS PCR products of such libraries.</jats:p>

Item Type: Article
Uncontrolled Keywords: Chromosomes, Artificial, Yeast; Animals; Mice, Inbred C57BL; Mice; Chromosome Mapping; Restriction Mapping; Crosses, Genetic; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Genomic Library; Genetic Linkage
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 02 Aug 2017 11:49
Last Modified: 15 Mar 2022 00:50
URI: http://repository.essex.ac.uk/id/eprint/11067

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