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Using hippocampal microRNA expression differences between mouse inbred strains to characterise miRNA function

Parsons, Michael J and Grimm, Christina H and Paya-Cano, Jose L and Sugden, Karen and Nietfeld, Wilfried and Lehrach, Hans and Schalkwyk, Leonard C (2008) 'Using hippocampal microRNA expression differences between mouse inbred strains to characterise miRNA function.' Mammalian Genome, 19 (7-8). pp. 552-560. ISSN 0938-8990

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Micro-RNAs (miRNAs) are short, single-stranded, noncoding RNAs that are involved in the regulation of protein-coding genes at the level of messenger RNA (mRNA). They are involved in the regulation of numerous traits, including developmental timing, apoptosis, immune function, and neuronal development. To better understand how the expression of the miRNAs themselves is regulated, we looked for miRNA expression differences among four mouse inbred strains, A/J, BALB/cJ, C57BL/6J, and DBA/2J, in one tissue, the hippocampus. A total of 166 miRNA RT-PCR assays were used to screen RNA pools for each strain. Twenty miRNA species that were markedly different between strains were further investigated using eight individual samples per strain, and 11 miRNAs showed significant differences across strains (p < 0.05). This is the first observation of miRNA expression differences across inbred mice strains. We conducted an in silico correlation analysis of the expression of these differentially expressed miRNAs with phenotype data and mRNA expression to better characterise the effects of these miRNAs on both phenotype and the regulation of mRNA expression. This approach has allowed us to nominate miRNAs that have potential roles in anxiety, exploration, and learning and memory. © 2008 Springer Science+Business Media, LLC.

Item Type: Article
Uncontrolled Keywords: Hippocampus; Animals; Mice, Inbred Strains; Mice; MicroRNAs; RNA, Messenger; Analysis of Variance; Species Specificity; Gene Expression Regulation; Sex Characteristics; Phenotype; Female; Male; Metabolic Networks and Pathways; Genetic Testing
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Dec 2014 18:31
Last Modified: 15 Jan 2022 00:49

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