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Panel of microsatellite markers for whole-genome scans and radiation hybrid mapping and a mouse family tree

Schalkwyk, LC and Jung, M and Daser, A and Weiher, M and Walter, J and Himmelbauer, H and Lehrach, H (1999) 'Panel of microsatellite markers for whole-genome scans and radiation hybrid mapping and a mouse family tree.' Genome Research, 9 (9). 878 - 887. ISSN 1088-9051

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To facilitate whole-genome scan experiments, we selected a panel of 128 microsatellite markers on the basis of spacing and polymorphism in the strains DBA/2 BALB/c, AKR, C57BL/6, C57BL/10, A/J, C3H, 129/J, SJL/J, JFI, and PWB. Many of the primer pairs were redesigned for better performance. The last four strains were not characterized previously using these markers. JFI and PWB are particularly interesting for intersubspecific crosses offering high polymorphism. We provide allele size data for the markers on these strains and add them to the emerging radiation hybrid framework map, which is not continuous except for chromosome 17 and 13. Information on the interrelationships of strains is useful both because of the importance of polymorphism in designing crosses and the background in assessing phenotypes. Microsatellites offer a widely dispersed, selectively neutral set of characters that lends itself conceptually to parsimony methods of analysis. The microsatellite allele size data were recoded as binary discrete characters in such a way that adjacent sizes differ by one step. Trees were generated using a Wagner parsimony method. As expected, the non-Mus domesticus strains, PWB (musculus) and JFI (molossinus), are excluded from the domesticus strains. Among the domesticus strains, C57BL/6 and C57BL/10 (derived from the same founding pair] form a strongly supported group, as do C3H, A/J, and BALB/c (derived from the Bagg albino stock). No unique branching order for SJL/J, AKR, and DBA/2 is strongly supported, which may reflect a complicated history. Strain 129/J is clearly placed as the most deeply diverged of the domesticus strains represented.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 02 Aug 2017 08:40
Last Modified: 20 Aug 2019 10:15

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