Research Repository

Panel of Microsatellite Markers for Whole-Genome Scans and Radiation Hybrid Mapping and a Mouse Family Tree

Schalkwyk, Leonard C and Jung, Martin and Daser, Angelika and Weiher, Michael and Walter, Jörn and Himmelbauer, Heinz and Lehrach, Hans (1999) 'Panel of Microsatellite Markers for Whole-Genome Scans and Radiation Hybrid Mapping and a Mouse Family Tree.' Genome Research, 9 (9). pp. 878-887. ISSN 1088-9051

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<jats:p>To facilitate whole-genome scan experiments, we selected a panel of 128 microsatellite markers on the basis of spacing and polymorphism in the strains DBA/2, BALB/c, AKR, C57BL/6, C57BL/10, A/J, C3H, 129/J, SJL/J, JF1, and PWB. Many of the primer pairs were redesigned for better performance. The last four strains were not characterized previously using these markers. JF1 and PWB are particularly interesting for intersubspecific crosses offering high polymorphism. We provide allele size data for the markers on these strains and add them to the emerging radiation hybrid framework map, which is not continuous except for chromosome 17 and 13. Information on the interrelationships of strains is useful both because of the importance of polymorphism in designing crosses and the background in assessing phenotypes. Microsatellites offer a widely dispersed, selectively neutral set of characters that lends itself conceptually to parsimony methods of analysis. The microsatellite allele size data were recoded as binary discrete characters in such a way that adjacent sizes differ by one step. Trees were generated using a Wagner parsimony method. As expected, the non-<jats:italic>Mus domesticus</jats:italic> strains, PWB (<jats:italic>musculus</jats:italic>) and JF1 (<jats:italic>molossinus</jats:italic>), are excluded from the <jats:italic>domesticus</jats:italic> strains. Among the <jats:italic>domesticus</jats:italic> strains, C57BL/6 and C57BL/10 (derived from the same founding pair) form a strongly supported group, as do C3H, A/J, and BALB/c (derived from the Bagg albino stock). No unique branching order for SJL/J, AKR, and DBA/2 is strongly supported, which may reflect a complicated history. Strain 129/J is clearly placed as the most deeply diverged of the<jats:italic>domesticus</jats:italic> strains represented.</jats:p>

Item Type: Article
Uncontrolled Keywords: Animals; Mice, Inbred Strains; Mice; DNA Primers; Physical Chromosome Mapping; Phylogeny; Microsatellite Repeats; Polymorphism, Genetic; Alleles; Genome; Models, Genetic
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 02 Aug 2017 08:40
Last Modified: 18 Aug 2022 11:41

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