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Overexpression of Plastid Transketolase in Tobacco Results in a Thiamine Auxotrophic Phenotype

Khozaei, M and Fisk, S and Lawson, T and Gibon, Y and Sulpice, R and Stitt, M and Lefebvre, SC and Raines, CA (2015) 'Overexpression of Plastid Transketolase in Tobacco Results in a Thiamine Auxotrophic Phenotype.' The Plant Cell, 27 (2). pp. 432-447. ISSN 1040-4651

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To investigate the effect of increased plastid transketolase on photosynthetic capacity and growth, tobacco (Nicotiana tabacum) plants with increased levels of transketolase protein were produced. This was achieved using a cassette composed of a full-length Arabidopsis thaliana transketolase cDNA under the control of the cauliflower mosaic virus 35S promoter. The results revealed a major and unexpected effect of plastid transketolase overexpression as the transgenic tobacco plants exhibited a slow-growth phenotype and chlorotic phenotype. These phenotypes were complemented by germinating the seeds of transketolase-overexpressing lines in media containing either thiamine pyrophosphate or thiamine. Thiamine levels in the seeds and cotyledons were lower in transketolase-overexpressing lines than in wild-type plants. When transketolase-overexpressing plants were supplemented with thiamine or thiamine pyrophosphate throughout the life cycle, they grew normally and the seed produced from these plants generated plants that did not have a growth or chlorotic phenotype. Our results reveal the crucial importance of the level of transketolase activity to provide the precursor for synthesis of intermediates and to enable plants to produce thiamine and thiamine pyrophosphate for growth and development. The mechanism determining transketolase protein levels remains to be elucidated, but the data presented provide evidence that this may contribute to the complex regulatory mechanisms maintaining thiamine homeostasis in plants.

Item Type: Article
Uncontrolled Keywords: Plastids; Plants, Genetically Modified; Arabidopsis; Tobacco; Seeds; Plant Leaves; Cotyledon; Carbon Dioxide; Carbon; Propanols; Shikimic Acid; Thiamine Pyrophosphate; Transketolase; Carbohydrates; Xylose; Thiamine; Amino Acids, Aromatic; RNA, Messenger; Genetic Complementation Test; Germination; Photosynthesis; Gene Expression Regulation, Plant; Phenotype; Models, Biological
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 23 Feb 2015 14:16
Last Modified: 15 Jan 2022 00:18

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