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Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.

Moon, RW and Hall, J and Rangkuti, F and Ho, YS and Almond, N and Mitchell, GH and Pain, A and Holder, AA and Blackman, MJ (2013) 'Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.' Proceedings of the National Academy of Sciences of the United States of America, 110 (2). pp. 531-536. ISSN 1091-6490

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Research into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.

Item Type: Article
Uncontrolled Keywords: Erythrocytes; Animals; Macaca fascicularis; Humans; Plasmodium knowlesi; DNA Primers; Cryopreservation; Culture Techniques; Cloning, Molecular; Polymerase Chain Reaction; Sequence Analysis, DNA; Adaptation, Biological; Species Specificity; Base Sequence; Genotype; Molecular Sequence Data
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 23 Mar 2015 13:16
Last Modified: 15 Jan 2022 01:05

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