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Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy

Kimata, Naoki and Reeves, Philip J and Smith, Steven O (2015) 'Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy.' Journal of Magnetic Resonance, 253. pp. 111-118. ISSN 1090-7807

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Abstract G protein-coupled receptors (GPCRs) span cell membranes with seven transmembrane helices and respond to a diverse array of extracellular signals. Crystal structures of GPCRs have provided key insights into the architecture of these receptors and the role of conserved residues. However, the question of how ligand binding induces the conformational changes that are essential for activation remains largely unanswered. Since the extracellular sequences and structures of GPCRs are not conserved between receptor subfamilies, it is likely that the initial molecular triggers for activation vary depending on the specific type of ligand and receptor. In this article, we describe NMR studies on the rhodopsin subfamily of GPCRs and propose a mechanism for how retinal isomerization switches the receptor to the active conformation. These results suggest a general approach for determining the triggers for activation in other GPCR subfamilies using NMR spectroscopy.

Item Type: Article
Uncontrolled Keywords: Solid-state NMR spectroscopy; G protein-coupled receptor; Magic angle spinning
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 14 Apr 2015 08:42
Last Modified: 15 Jan 2022 00:30

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