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Seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient

Smith, CJ and Dong, LF and Wilson, J and Stott, A and Osborn, AM and Nedwell, DB (2015) 'Seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient.' Frontiers in Microbiology, 6 (542). ISSN 1664-302X

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Abstract

This research investigated spatial-temporal variation in benthic bacterial community structure, rates of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) processes and abundances of corresponding genes and transcripts at three sites ? the estuary-head, mid-estuary and the estuary mouth along the nitrate gradient of the Colne estuary over an annual cycle. Denitrification rates declined down the estuary, while DNRA rates were higher at the estuary head and middle than the estuary mouth. In four out of the six two-monthly time-points, rates of DNRA were greater than denitrification at each site. Abundance of gene markers for nitrate-reduction (nitrate reductase narG and napA), denitrification (nitrite reductase nirS) and DNRA (DNRA nitrite reductase nrfA) declined along the estuary with significant relationships between denitrification and nirS abundance, and DNRA and nrfA abundance. Spatially, rates of denitrification, DNRA and corresponding functional gene abundances decreased along the estuary. However, temporal correlations between rate processes and functional gene and transcript abundances were not observed.

Item Type: Article
Uncontrolled Keywords: Denitrification, DNRA, esturine sediments, narG, nrfA, (RT)-Q-PCR, Napa, NIRS
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 20 May 2015 09:22
Last Modified: 25 Sep 2019 09:15
URI: http://repository.essex.ac.uk/id/eprint/13832

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