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Blobs and curves: object-based colocalisation for plant cells

Nelson, CJ and Duckney, P and Hawkins, TJ and Deeks, MJ and Laissue, PP and Hussey, PJ and Obara, B (2014) 'Blobs and curves: object-based colocalisation for plant cells.' Functional Plant Biology, 42 (5). 471 - 485. ISSN 0310-7841

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Abstract

Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.

Item Type: Article
Uncontrolled Keywords: actin-binding proteins, bioimage informatics, fluorescence microscopy, image analysis, mitochondrial trafficking, plant science
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 09 Jul 2015 10:35
Last Modified: 14 Oct 2019 17:23
URI: http://repository.essex.ac.uk/id/eprint/14084

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