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Single molecule techniques in DNA repair: A primer

Hughes, Craig D and Simons, Michelle and Mackenzie, Cassidy E and Van Houten, Bennett and Kad, Neil M (2014) 'Single molecule techniques in DNA repair: A primer.' DNA Repair, 20. pp. 2-13. ISSN 1568-7864

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A powerful new approach has become much more widespread and offers insights into aspects of DNA repair unattainable with billions of molecules. Single molecule techniques can be used to image, manipulate or characterize the action of a single repair protein on a single strand of DNA. This allows search mechanisms to be probed, and the effects of force to be understood. These physical aspects can dominate a biochemical reaction, where at the ensemble level their nuances are obscured. In this paper we discuss some of the many technical advances that permit study at the single molecule level. We focus on DNA repair to which these techniques are actively being applied. DNA repair is also a process that encompasses so much of what single molecule studies benefit - searching for targets, complex formation, sequential biochemical reactions and substrate hand-off to name just a few. We discuss how single molecule biophysics is poised to transform our understanding of biological systems, in particular DNA repair. © 2014.

Item Type: Article
Uncontrolled Keywords: DNA repair; Single molecule; FRET, Imaging; Laser tweezers; Magnetic tweezers; AFM
Subjects: Q Science > Q Science (General)
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 08 Jul 2015 10:24
Last Modified: 15 Jan 2022 00:32

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