McKew, Boyd A and Metodieva, Gergana and Raines, Christine A and Metodiev, Metodi V and Geider, Richard J (2015) 'Acclimation of<i>E</i><i>miliania huxleyi</i>(1516) to nutrient limitation involves precise modification of the proteome to scavenge alternative sources of N and P.' Environmental Microbiology, 17 (10). pp. 4050-4062. ISSN 1462-2912

Preview

Text McKew_et_al-2015-Environmental_Microbiology.pdf - Published Version Available under License Creative Commons Attribution. Download (671kB) | Preview

Abstract

Limitation of marine primary production by the availability of nitrogen or phosphorus is common. Emiliania huxleyi, a ubiquitous phytoplankter that plays key roles in primary production, calcium carbonate precipitation and production of dimethyl sulfide, often blooms in mid-latitude at the beginning of summer when inorganic nutrient concentrations are low. To understand physiological mechanisms that allow such blooms, we examined how the proteome of E. huxleyi (strain 1516) responds to N and P limitation. We observed modest changes in much of the proteome despite large physiological changes (e.g. cellular biomass, C, N and P) associated with nutrient limitation of growth rate. Acclimation to nutrient limitation did however involve significant increases in the abundance of transporters for ammonium and nitrate under N limitation and for phosphate under P limitation. More notable were large increases in proteins involved in the acquisition of organic forms of N and P, including urea and amino acid/polyamine transporters and numerous C-N hydrolases under N limitation and a large upregulation of alkaline phosphatase under P limitation. This highly targeted reorganization of the proteome towards scavenging organic forms of macronutrients gives unique insight into the molecular mechanisms that underpin how E. huxleyi has found its niche to bloom in surface waters depleted of inorganic nutrients.

Item Type:	Article
Uncontrolled Keywords:	Phytoplankton; Calcium Carbonate; Phosphates; Sulfides; Nitrogen; Phosphorus; Polyamines; Urea; Alkaline Phosphatase; Amino Acids; Proteome; Biomass; Acclimatization; Haptophyta
Subjects:	G Geography. Anthropology. Recreation > GC Oceanography
Divisions:	Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of
SWORD Depositor:	Elements
Depositing User:	Elements
Date Deposited:	30 Jun 2015 14:52
Last Modified:	11 Apr 2022 18:00
URI:	http://repository.essex.ac.uk/id/eprint/14172

Actions (login required)

View Item