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A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii

Defosse, Tatiana A and Melin, Céline and Obando Montoya, Erika J and Lanoue, Arnaud and Foureau, Emilien and Glévarec, Gaëlle and Oudin, Audrey and Simkin, Andrew J and Crèche, Joël and Atehortùa, Lucia and Giglioli-Guivarc’h, Nathalie and Clastre, Marc and Courdavault, Vincent and Papon, Nicolas (2014) 'A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii.' Journal of Biotechnology, 180. pp. 37-42. ISSN 0168-1656

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Abstract

The biotechnological potential of C. guilliermondii is now well established. This yeast species currently benefits from the availability of a convenient molecular toolbox including recipient strains, selectable markers and optimized transformation protocols. However, the number of expression systems for biotechnological applications in C. guilliermondii remains limited. We have therefore developed and characterized a new series of versatile controllable expression vectors for this yeast. While previous studies firmly demonstrated that knock-out systems represent efficient genetic strategies to interrupt yeast biochemical pathways at a specific step in C. guilliermondii, the set of expression plasmids described in this study will provide new powerful opportunities to boost homologous or heterologous biosynthetic routes by fine controlled over-expression approaches. © 2014 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Constitutive promoter; Inducible promoter; Repressible promoter; Expression vectors; Candida guilliermondii
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 06 Jul 2015 14:14
Last Modified: 15 Jan 2022 00:32
URI: http://repository.essex.ac.uk/id/eprint/14239

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