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Standards for plant synthetic biology: a common syntax for exchange of <scp>DNA</scp> parts

Patron, Nicola J and Orzaez, Diego and Marillonnet, Sylvestre and Warzecha, Heribert and Matthewman, Colette and Youles, Mark and Raitskin, Oleg and Leveau, Aymeric and Farré, Gemma and Rogers, Christian and Smith, Alison and Hibberd, Julian and Webb, Alex AR and Locke, James and Schornack, Sebastian and Ajioka, Jim and Baulcombe, David C and Zipfel, Cyril and Kamoun, Sophien and Jones, Jonathan DG and Kuhn, Hannah and Robatzek, Silke and Van Esse, H Peter and Sanders, Dale and Oldroyd, Giles and Martin, Cathie and Field, Rob and O'Connor, Sarah and Fox, Samantha and Wulff, Brande and Miller, Ben and Breakspear, Andy and Radhakrishnan, Guru and Delaux, Pierre‐Marc and Loqué, Dominique and Granell, Antonio and Tissier, Alain and Shih, Patrick and Brutnell, Thomas P and Quick, W Paul and Rischer, Heiko and Fraser, Paul D and Aharoni, Asaph and Raines, Christine and South, Paul F and Ané, Jean‐Michel and Hamberger, Björn R and Langdale, Jane and Stougaard, Jens and Bouwmeester, Harro and Udvardi, Michael and Murray, James AH and Ntoukakis, Vardis and Schäfer, Patrick and Denby, Katherine and Edwards, Keith J and Osbourn, Anne and Haseloff, Jim (2015) 'Standards for plant synthetic biology: a common syntax for exchange of <scp>DNA</scp> parts.' New Phytologist, 208 (1). pp. 13-19. ISSN 0028-646X

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Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Item Type: Article
Uncontrolled Keywords: cloning; DNA assembly; genetic syntax; GoldenGate; synthetic biology; Type IIS restriction endonucleases
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 15 Jul 2015 08:21
Last Modified: 11 Apr 2022 13:28
URI: http://repository.essex.ac.uk/id/eprint/14334

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