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Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Patron, NJ and Orzaez, D and Marillonnet, S and Warzecha, H and Matthewman, C and Youles, M and Raitskin, O and Leveau, A and Farré, G and Rogers, C and Smith, A and Hibberd, J and Webb, AAR and Locke, J and Schornack, S and Ajioka, J and Baulcombe, DC and Zipfel, C and Kamoun, S and Jones, JDG and Kuhn, H and Robatzek, S and Van Esse, HP and Sanders, D and Oldroyd, G and Martin, C and Field, R and O'Connor, S and Fox, S and Wulff, B and Miller, B and Breakspear, A and Radhakrishnan, G and Delaux, PM and Loqué, D and Granell, A and Tissier, A and Shih, P and Brutnell, TP and Quick, WP and Rischer, H and Fraser, PD and Aharoni, A and Raines, C and South, PF and Ané, JM and Hamberger, BR and Langdale, J and Stougaard, J and Bouwmeester, H and Udvardi, M and Murray, JAH and Ntoukakis, V and Schäfer, P and Denby, K and Edwards, KJ and Osbourn, A and Haseloff, J (2015) 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts.' New Phytologist, 208 (1). 13 - 19. ISSN 0028-646X

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Abstract

© 2015 New Phytologist Trust. Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 15 Jul 2015 08:21
Last Modified: 04 Feb 2019 11:16
URI: http://repository.essex.ac.uk/id/eprint/14334

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