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The reaction of oxyhemoglobin with nitric oxide: EPR evidence for an iron(III)-nitrate intermediate

Silaghi-Dumitrescu, R and Scurtu, F and Mason, MG and Svistunenko, DA and Wilson, MT and Cooper, CE (2015) 'The reaction of oxyhemoglobin with nitric oxide: EPR evidence for an iron(III)-nitrate intermediate.' Inorganica Chimica Acta, 436. 179 - 183. ISSN 0020-1693

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Abstract

© 2015 Elsevier B.V. All rights reserved. The reaction of oxyhemoglobin with nitric oxide is known to occur in vivo, with direct medical and pathophysiological relevance. The mechanism has been proposed to involve a transient iron(III)-peroxynitrite intermediate, the formation of which would be rate-limiting and the decay of which would yield iron(III)-aqua/iron(III)-hydroxo hemoglobin. Reported here are rapid-freeze-quench EPR (RFQ-EPR) spectroscopy data on the reaction of oxyhemoglobin with nitric oxide; no direct evidence is seen for an iron(III)-peroxynitrite intermediate. These findings are consistent with theoretical considerations according to which such an intermediate does form but is too short-lived to be detectable. Instead, iron(III) low-spin and high-spin transient species are detected in the RFQ-EPR experiments, which can be explained as arising from interactions of nitrate with methemoglobin.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 18 Aug 2015 12:49
Last Modified: 14 Oct 2019 17:23
URI: http://repository.essex.ac.uk/id/eprint/14560

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