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MD and NMR studies of α-bungarotoxin surface accessibility

Venditti, V and Bernini, A and De Simone, A and Spiga, O and Prischi, F and Niccolai, N (2007) 'MD and NMR studies of α-bungarotoxin surface accessibility.' Biochemical and Biophysical Research Communications, 356 (1). 114 - 117. ISSN 0006-291X

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Abstract

Protein surface accessibility represents a dimension of structural biology which has not been discussed in details so far, in spite of its fundamental role in controlling the molecular recognition process. In the present report the surface accessibility of α-bungarotoxin, a small and well characterized protein, has been investigated by analyzing its interaction with solvent and paramagnetic molecules in an integrated way. The presence of strong hydration sites, identified by a combined analysis of MD simulation and NMR results, seems to prevent the access of Gd(III)DTPA-BMA to the protein surface. On the contrary, the limited hydration of the α-bungarotoxin active site favors frequent encounters between the paramagnetic probe and the protein in the latter region. All the data obtained here for α-bungarotoxin suggest that shape and stability of the solvation shell control its surface accessibility and, hence, intermolecular interactions in a way which could be common to many other proteins. © 2007 Elsevier Inc. All rights reserved.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Filippo Prischi
Date Deposited: 04 Feb 2016 11:53
Last Modified: 17 Aug 2017 17:33
URI: http://repository.essex.ac.uk/id/eprint/14834

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