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Of the vulnerability of orphan complex proteins: The case study of the E. coli IscU and IscS proteins

Prischi, F and Pastore, C and Carroni, M and Iannuzzi, C and Adinolfi, S and Temussi, P and Pastore, A (2010) 'Of the vulnerability of orphan complex proteins: The case study of the E. coli IscU and IscS proteins.' Protein Expression and Purification, 73 (2). 161 - 166. ISSN 1046-5928

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Abstract

IscS and IscU, the two central protein components of the iron sulfur cluster assembly machinery, form a complex that is still relatively poorly characterized. In an attempt to standardize the purification of these proteins for structural studies we have developed a protocol to produce them individually in high concentration and purity. We show that IscS is a rather robust protein as long as it is produced in a PLP loaded form and that this co-factor is essential for fold stability and enzyme activity. In contrast to previous evidence, we also propose that, in contrast with previous evidence, IscU is a thermodynamically stable protein with a well defined fold but, when produced in isolation, is a 'complex-orphan protein' that is prone to unfolding if not stabilised by a co-factor or a protein partner. Our work will facilitate further structural and functional studies of these proteins and eventually lead to a better understanding of the whole machinery. © 2010 Elsevier Inc. All rights reserved.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Filippo Prischi
Date Deposited: 22 Dec 2015 16:10
Last Modified: 04 Sep 2019 06:15
URI: http://repository.essex.ac.uk/id/eprint/14841

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