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Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c’

Ghafoor, Dlzar D and Kekilli, Demet and Abdullah, Gaylany H and Dworkowski, Florian SN and Hassan, Hamid G and Wilson, Michael T and Strange, Richard W and Hough, Michael A (2015) 'Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c’.' JBIC Journal of Biological Inorganic Chemistry, 20 (6). pp. 949-956. ISSN 0949-8257

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Abstract

Cytochromes c', that occur in methanotrophic, denitrifying and photosynthetic bacteria, form unusual proximal penta-coordinate NO complexes via a hexa-coordinate distal NO intermediate. Their NO binding properties are similar to those of the eukaryotic NO sensor, soluble guanylate cyclase, for which they provide a valuable structural model. Previous studies suggested that hydrogen bonding between the displaced proximal histidine (His120) ligand (following its dissociation from heme due to trans effects from the distally bound NO) and a conserved aspartate residue (Asp121) could play a key role in allowing proximal NO binding to occur. We have characterized three variants of Alcaligenes xylosoxidans cytochrome c' (AXCP) where Asp121 has been replaced by Ala, Ile and Gln, respectively. In all variants, hydrogen bonding between residue 121 and His120 is abolished yet 5-coordinate proximal NO species are still formed. Our data therefore demonstrate that the His120-Asp121 bond is not essential for proximal NO binding although it likely provides an energy minimum for the displaced His ligand. All variants have altered proximal pocket structure relative to native AXCP.

Item Type: Article
Uncontrolled Keywords: Cytochrome; Nitric oxide; X-ray crystallography; Resonance Raman; Spectroscopy; Ligand binding
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Sep 2015 14:31
Last Modified: 18 Aug 2022 11:07
URI: http://repository.essex.ac.uk/id/eprint/14941

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