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Structurally Driven Selection of Human Hepatitis C Virus Mimotopes

Spiga, Ottavia and Padula, Maria G and Scarselli, Maria and Ciutti, Arianna and Bernini, Andrea and Venditti, Vincenzo and Prischi, Filippo and Falciani, Chiara and Lozzi, Luisa and Bracci, Luisa and Valensin, Piero E and Caudai, Cinzia and Niccolai, Neri (2006) 'Structurally Driven Selection of Human Hepatitis C Virus Mimotopes.' Antiviral Therapy, 11 (7). pp. 917-922. ISSN 1359-6535

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Abstract

<jats:p> A structural genomics approach is proposed for the development of new diagnostic kits. It combines molecular modelling, peptide synthesis and immunological tests. The preliminary step is the development of a reliable three-dimensional structure of an immunodominant protein of the target pathogenic organism using the various bioinformatic strategies that are now available to structural biologists. Once the protein structure is obtained, the most surface-exposed fragments with minimal sequence variability among the different strains reported in the genomic data bank are reproduced synthetically as linear peptides. These peptides are then tested for immunoreactivity with the plasma of infected patients to determine whether the synthetic molecules have antigenic activity and can therefore be used to detect infecting agents. This structurally driven selection of mimotopes was successfully performed for the human hepatitis C virus, as five peptides that specifically interact with the plasma of HCV-infected patients were identified solely on the basis of the three-dimensional structure predicted for the E2 homodimer of the 1a viral subtype. A similar approach could easily be extended to a large variety of immunogenic proteins from other pathogenic organisms. </jats:p>

Item Type: Article
Uncontrolled Keywords: Humans; Hepacivirus; Hepatitis C; Peptides; Viral Proteins; Viral Envelope Proteins; Immune Sera; Enzyme-Linked Immunosorbent Assay; Sequence Alignment; Species Specificity; Genes, Viral; Models, Molecular; Molecular Sequence Data; Selection, Genetic
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 04 Feb 2016 13:02
Last Modified: 13 May 2022 09:56
URI: http://repository.essex.ac.uk/id/eprint/15680

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