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The effect of an induced benzene plume on microbial communities in a groundwater aquifer

Aganbi, E and Medina, AA and Fahy, A and McGenity, TJ and Ball, AS (2014) The effect of an induced benzene plume on microbial communities in a groundwater aquifer. In: Proceedings of the 24th Annual International Conference on Water, Energy & Air, ? - ?, San Diego.

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Abstract

This study investigated the degradation of a benzene plume by indigenous microbial communities in sediments and groundwater from the SIReN (Site for Innovative Research in Natural Attenuation). A sandbox mesocosm system and microcosms were inoculated with SIReN sediments/groundwater and spiked separately with plume (starting concentration of 1000 mg/l-1) and various concentrations of benzene (5 ? 500 mg/l-1). Benzene plume was degraded almost at the point of entry, while in aerobic microcosms benzene was degraded to varying degrees by day 6 compared to killed controls. DGGE analysis of 16S rRNA gene (rDNA) allowed the detection of species closely related to Rhodoferax ferrireducens (95% similarity) and Dechloromonas aromatica RCB (99% similarity). Other organisms detected were closely related to Methylotenera mobilis strain JLW8 (92 ? 96% similarity); Nitrosomonas halophila strain Nm 1 (97% similarity); Insolitispirillum peregrinum subsp. integrum strain LMG 5407 (99% similarity); and Lactococcus lactis strain SWU15983 (97% similarity). Our results demonstrated that benzene contamination had a profound impact on in situ microbial community structure and composition. Furthermore, the abundance of organisms closely related to methylotrophic bacteria may be indicative of a more ?active? role in bioremediation for this group than previously known. Results from this study add to available information on the identity of benzene-degrading taxa that may be useful for the bioremediation of hydrocarbon-contaminated aquifers under microaerophilic conditions.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Published proceedings: _not provided_
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 01 Jul 2016 14:43
Last Modified: 25 Sep 2019 10:16
URI: http://repository.essex.ac.uk/id/eprint/17151

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