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Modular design of the selectivity filter pore loop in a novel family of prokaryotic inward rectifier' (NirBac) channels

Zubcevic, Lejla and Wang, Shizhen and Bavro, Vassiliy N and Lee, Sun-Joo and Nichols, Colin G and Tucker, Stephen J (2015) 'Modular design of the selectivity filter pore loop in a novel family of prokaryotic inward rectifier' (NirBac) channels.' Scientific Reports, 5 (1). 15305-. ISSN 2045-2322

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Potassium channels exhibit a modular design with distinct structural and functional domains; in particular, a highly conserved pore-loop sequence that determines their ionic selectivity. We now report the functional characterisation of a novel group of functionally non-selective members of the prokaryotic inward rectifier' subfamily of K + channels. These channels share all the key structural domains of eukaryotic and prokaryotic Kir/KirBac channels, but instead possess unique pore-loop selectivity filter sequences unrelated to any other known ionic selectivity filter. The strikingly unusual architecture of these NirBac' channels defines a new family of functionally non-selective ion channels, and also provides important insights into the modular design of ion channels, as well as the evolution of ionic selectivity within this superfamily of tetrameric cation channels.

Item Type: Article
Uncontrolled Keywords: Myxococcales; Bacterial Proteins; Potassium Channels, Inwardly Rectifying; Recombinant Proteins; Evolution, Molecular; Phylogeny; Ion Transport; Amino Acid Sequence; Protein Structure, Tertiary; Models, Molecular; Molecular Sequence Data
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 06 Oct 2016 16:21
Last Modified: 06 Jan 2022 14:40

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