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Sequential structural changes in rhodopsin occurring upon photoactivation

Kimata, N and Pope, A and Rashid, D and Reeves, PJ and Smith, SO (2015) 'Sequential structural changes in rhodopsin occurring upon photoactivation.' In: UNSPECIFIED, (ed.) UNSPECIFIED UNSPECIFIED, 159 - 171.

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Abstract

© Springer Science+Business Media New York 2015. We describe the use of solid-state magic angle spinning NMR spectroscopy for characterizing the structure and dynamics of dark, inactive rhodopsin and the active metarhodopsin II intermediate. Solid-state NMR spectroscopy is well suited for structural measurements in both detergent micelles and membrane bilayer environments. We fi rst outline the methods for large-scale production of stable, functional rhodopsin containing 13 C- and 15 N-labeled amino acids. The expression methods make use of eukaryotic HEK293S cell lines that produce correctly folded, fully functional receptors. We subsequently describe the basic methods used for solid-state magic angle spinning NMR measurements of chemical shifts and dipolar couplings, which provide information on rhodopsin structure and dynamics, and describe the use of low-temperature methods to trap the active metarhodopsin II intermediate.

Item Type: Book Section
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 11 Dec 2016 10:49
Last Modified: 07 Oct 2019 11:15
URI: http://repository.essex.ac.uk/id/eprint/18468

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