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Simultaneous stimulation of sedoheptulose 1,7‐bisphosphatase, fructose 1,6‐bisphophate aldolase and the photorespiratory glycine decarboxylase‐H protein increases CO2 assimilation, vegetative biomass and seed yield in Arabidopsis

Simkin, AJ and Lopez-Calcagno, PE and Davey, PA and Headland, LR and Lawson, T and Timm, S and Bauwe, H and Raines, CA (2017) 'Simultaneous stimulation of sedoheptulose 1,7‐bisphosphatase, fructose 1,6‐bisphophate aldolase and the photorespiratory glycine decarboxylase‐H protein increases CO2 assimilation, vegetative biomass and seed yield in Arabidopsis.' Plant Biotechnology Journal, 15 (7). 805 - 816. ISSN 1467-7644

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Abstract

In this paper we have altered the levels of three different enzymes involved in the Calvin Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7-bisphosphatase (SBPase), fructose 1,6-bisphophate aldolase (FBPA) and the glycine decarboxylase H-protein (GDC-H) gene identified as targets to improve photosynthesis based on previous studies. Here, we show that increasing the levels of the three corresponding proteins, either independently or in combination, significantly increases the quantum efficiency of PSII. Furthermore, photosynthetic measurements demonstrated an increase in the maximum efficiency of CO2 fixation in lines over-expressing SBPase and FBPA. Moreover, the co-expression of GDC-H with SBPase and FBPA resulted in a cumulative positive impact on leaf area and biomass. Finally, further analysis of transgenic lines revealed a cumulative increase of seed yield in SFH lines grown in high light. These results demonstrate the potential of multigene-stacking for improving the productivity of food and energy crops.

Item Type: Article
Uncontrolled Keywords: Photosynthesis; Glycine Decarboxylase H protein; SBPase; FBPaldolase; chlorophyll fluorescence imaging; transgenic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Humanities > International Academy
Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 14 Dec 2016 19:55
Last Modified: 18 Sep 2018 12:15
URI: http://repository.essex.ac.uk/id/eprint/18612

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