Research Repository

Yeast Golden Gate (yGG) for the Efficient Assembly of <i>S. cerevisiae</i> Transcription Units

Agmon, Neta and Mitchell, Leslie A and Cai, Yizhi and Ikushima, Shigehito and Chuang, James and Zheng, Allen and Choi, Woo-Jin and Martin, J Andrew and Caravelli, Katrina and Stracquadanio, Giovanni and Boeke, Jef D (2015) 'Yeast Golden Gate (yGG) for the Efficient Assembly of <i>S. cerevisiae</i> Transcription Units.' ACS Synthetic Biology, 4 (7). pp. 853-859. ISSN 2161-5063

Image (Graphical abstract)

Download (35kB) | Preview


We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for the yeast Saccharomyces cerevisiae, in a method we call yeast Golden Gate (yGG). yGG allows for the easy assembly of TUs consisting of promoters (PRO), coding sequences (CDS), and terminators (TER). Carefully designed overhangs exposed by digestion with a type IIS restriction enzyme enable virtually seamless assembly of TUs that, in principle, contain all of the information necessary to express a gene of interest in yeast. We also describe a versatile set of yGG acceptor vectors to be used for TU assembly. These vectors can be used for low or high copy expression of assembled TUs or integration into carefully selected innocuous genomic loci. yGG provides synthetic biologists and yeast geneticists with an efficient new means by which to engineer S. cerevisiae. (Figure Presented).

Item Type: Article
Uncontrolled Keywords: synthetic biology; DNA assembly; S. cerevisiae; transcription unit; yeast Golden Gate
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Computer Science and Electronic Engineering, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Jan 2017 12:22
Last Modified: 18 Aug 2022 11:32

Actions (login required)

View Item View Item