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Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units

Agmon, N and Mitchell, LA and Cai, Y and Ikushima, S and Chuang, J and Zheng, A and Choi, WJ and Martin, JA and Caravelli, K and Stracquadanio, G and Boeke, JD (2015) 'Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.' ACS Synthetic Biology, 4 (7). 853 - 859. ISSN 2161-5063

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Abstract

© 2015 American Chemical Society. We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for the yeast Saccharomyces cerevisiae, in a method we call yeast Golden Gate (yGG). yGG allows for the easy assembly of TUs consisting of promoters (PRO), coding sequences (CDS), and terminators (TER). Carefully designed overhangs exposed by digestion with a type IIS restriction enzyme enable virtually seamless assembly of TUs that, in principle, contain all of the information necessary to express a gene of interest in yeast. We also describe a versatile set of yGG acceptor vectors to be used for TU assembly. These vectors can be used for low or high copy expression of assembled TUs or integration into carefully selected innocuous genomic loci. yGG provides synthetic biologists and yeast geneticists with an effi cient new means by which to engineer S. cerevisiae. (Figure Presented).

Item Type: Article
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Computer Science and Electronic Engineering, School of
Depositing User: Giovanni Stracquadanio
Date Deposited: 16 Jan 2017 12:22
Last Modified: 30 Nov 2017 15:16
URI: http://repository.essex.ac.uk/id/eprint/18691

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