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Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes

Cai, Yizhi and Agmon, Neta and Choi, Woo Jin and Ubide, Alba and Stracquadanio, Giovanni and Caravelli, Katrina and Hao, Haiping and Bader, Joel S and Boeke, Jef D (2015) 'Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes.' Proceedings of the National Academy of Sciences, 112 (6). pp. 1803-1808. ISSN 0027-8424

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Abstract

<jats:title>Significance</jats:title> <jats:p>The advance of biotechnology opens up greater possibilities of bioterror and bioerror. Here, we propose multiplexed safeguard switches rooted in the development of foundational genomic, regulatory, and metabolic technologies. Safeguard switches can be regulated by submicromolar small molecule(s) and combined in a modular fashion. The resulting safeguard strains show high fitness and low reversion rates. Moreover, two distinct classes of safeguard switches are orthogonal, providing a potential fail-safe mechanism. The safeguard technologies provide a practical and generic approach to containing engineered microbes within defined laboratory and/or industrial environments, and can in principle be used in the field as well.</jats:p>

Item Type: Article
Uncontrolled Keywords: biotechnology; biosafety; Saccharomyces cerevisiae
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QD Chemistry
Divisions: Faculty of Science and Health
Faculty of Science and Health > Computer Science and Electronic Engineering, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Jan 2017 12:30
Last Modified: 15 Mar 2022 00:50
URI: http://repository.essex.ac.uk/id/eprint/18692

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