Research Repository

Total synthesis of a functional designer eukaryotic chromosome

Annaluru, N and Muller, H and Mitchell, LA and Ramalingam, S and Stracquadanio, G and Richardson, SM and Dymond, JS and Kuang, Z and Scheifele, LZ and Cooper, EM and Cai, Y and Zeller, K and Agmon, N and Han, JS and Hadjithomas, M and Tullman, J and Caravelli, K and Cirelli, K and Guo, Z and London, V and Yeluru, A and Murugan, S and Kandavelou, K and Agier, N and Fischer, G and Yang, K and Martin, JA and Bilgel, M and Bohutski, P and Boulier, KM and Capaldo, BJ and Chang, J and Charoen, K and Choi, WJ and Deng, P and DiCarlo, JE and Doong, J and Dunn, J and Feinberg, JI and Fernandez, C and Floria, CE and Gladowski, D and Hadidi, P and Ishizuka, I and Jabbari, J and Lau, CYL and Lee, PA and Li, S and Lin, D and Linder, ME and Ling, J and Liu, J and Liu, J and London, M and Henry, M and Mao, J and McDade, JE and McMillan, A and Moore, AM and Oh, WC and Ouyang, Y and Patel, R and Paul, M and Paulsen, LC and Qiu, J and Rhee, A and Rubashkin, MG and Soh, IY and Sotuyo, NE and Srinivas, V and Suarez, A and Wong, A and Wong, R and Xie, WR and Xu, Y and Yu, AT and Koszul, R and Bader, JS and Boeke, JD (2014) 'Total synthesis of a functional designer eukaryotic chromosome.' Science, 344 (6179). 55 - 58. ISSN 0036-8075

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Abstract

Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.

Item Type: Article
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Computer Science and Electronic Engineering, School of
Depositing User: Giovanni Stracquadanio
Date Deposited: 02 Feb 2017 15:11
Last Modified: 17 Aug 2017 17:20
URI: http://repository.essex.ac.uk/id/eprint/18695

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