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Near-complete backbone resonance assignments of acid-denatured human cytochrome c in dimethylsulfoxide: a prelude to studying interactions with phospholipids

Karsisiotis, AI and Deacon, OM and Macdonald, C and Blumenschein, TMA and Moore, GR and Worrall, JAR (2017) 'Near-complete backbone resonance assignments of acid-denatured human cytochrome c in dimethylsulfoxide: a prelude to studying interactions with phospholipids.' Biomolecular NMR Assignments, 11 (2). 165 - 168. ISSN 1874-2718

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Abstract

© 2017, Springer Science+Business Media Dordrecht. Human cytochrome c plays a central role in the mitochondrial electron transfer chain and in the intrinsic apoptosis pathway. Through the interaction with the phospholipid cardiolipin, cytochrome c triggers release of pro-apoptotic factors, including itself, from the mitochondrion into the cytosol of cells undergoing apoptosis. The cytochrome c/cardiolipin complex has been extensively studied through various spectroscopies, most recently with high-field solution and solid-state NMR spectroscopies, but there is no agreement between the various studies on key structural features of cytochrome c in its complex with cardiolipin. In the present study, we report backbone 1H, 13C, 15N resonance assignments of acid-denatured human cytochrome c in the aprotic solvent dimethylsulfoxide. These have led to the assignment of a reference 2D 1H-15N HSQC spectrum in which out of the 99 non-proline residues 87% of the backbone amides are assigned. These assignments are being used in an interrupted H/D exchange strategy to map the binding site of cardiolipin on human cytochrome c.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jonathan Worrall
Date Deposited: 19 Apr 2017 15:57
Last Modified: 30 Jan 2019 16:20
URI: http://repository.essex.ac.uk/id/eprint/19487

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