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Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Shen, Y and Wang, Y and Chen, T and Gao, F and Gong, J and Abramczyk, D and Walker, R and Zhao, H and Chen, S and Liu, W and Luo, Y and Müller, CA and Paul-Dubois-Taine, A and Alver, B and Stracquadanio, G and Mitchell, LA and Luo, Z and Fan, Y and Zhou, B and Wen, B and Tan, F and Wang, Y and Zi, J and Xie, Z and Li, B and Yang, K and Richardson, SM and Jiang, H and French, CE and Nieduszynski, CA and Koszul, R and Marston, AL and Yuan, Y and Wang, J and Bader, JS and Dai, J and Boeke, JD and Xu, X and Cai, Y and Yang, H (2017) 'Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.' Science, 355 (6329). ISSN 0036-8075

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Abstract

© 2017, American Association for the Advancement of Science. All rights reserved. Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels - including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis - to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QA Mathematics > QA76 Computer software
Q Science > QD Chemistry
Divisions: Faculty of Science and Health > Computer Science and Electronic Engineering, School of
Depositing User: Giovanni Stracquadanio
Date Deposited: 15 May 2017 09:51
Last Modified: 30 Jan 2019 16:20
URI: http://repository.essex.ac.uk/id/eprint/19590

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