Research Repository

"Perfect" designer chromosome v and behavior of a ring derivative

Xie, ZX and Li, BZ and Mitchell, LA and Wu, Y and Qi, X and Jin, Z and Jia, B and Wang, X and Zeng, BX and Liu, HM and Wu, XL and Feng, Q and Zhang, WZ and Liu, W and Ding, MZ and Li, X and Zhao, GR and Qiao, JJ and Cheng, JS and Zhao, M and Kuang, Z and Wang, X and Martin, JA and Stracquadanio, G and Yang, K and Bai, X and Zhao, J and Hu, ML and Lin, QH and Zhang, WQ and Shen, MH and Chen, S and Su, W and Wang, EX and Guo, R and Zhai, F and Guo, XJ and Du, HX and Zhu, JQ and Song, TQ and Dai, JJ and Li, FF and Jiang, GZ and Han, SL and Liu, SY and Yu, ZC and Yang, XN and Chen, K and Hu, C and Li, DS and Jia, N and Liu, Y and Wang, LT (2017) '"Perfect" designer chromosome v and behavior of a ring derivative.' Science, 355 (6329). ISSN 0036-8075

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Abstract

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis.We designed and de novo synthesized 536, 024-base pair chromosome synV in the "Build-A-Genome China" course.We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QA Mathematics > QA76 Computer software
Q Science > QD Chemistry
Divisions: Faculty of Science and Health > Computer Science and Electronic Engineering, School of
Depositing User: Giovanni Stracquadanio
Date Deposited: 15 May 2017 11:49
Last Modified: 05 Feb 2019 17:15
URI: http://repository.essex.ac.uk/id/eprint/19592

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