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The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)

Ferguson, RMW and Merrifield, DL and Harper, GM and Rawling, MD and Mustafa, S and Picchietti, S and Balcázar, JL and Davies, SJ (2010) 'The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus).' Journal of Applied Microbiology, 109 (3). 851 - 862. ISSN 0021-8847

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Abstract

Aim: To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). Methods and Results: Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10 7 CFU g -1 for 32 days. Ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici with an endogenous uncultured bacterium during a period of reverting to nonsupplemented feeding. Light microscopy revealed that gut integrity and leucocyte levels were unaffec ted by Ped. acidilactici; however, blood leucocyte levels and serum lysozyme activity were elevated after 14-days' feeding. No significant improvements in growth performance were observed at the end of the trial (day 32), but survival was significantly higher in the probiotic group. Conclusions: The study demonstrates that oral supplementation of Ped. acidilactici modulates intestinal bacterial communities in on-growing red tilapia and also stimulates some aspects of the nonspecific immune response. Significance and Impact of the study: To our knowledge this is the first study assessing the effects of probiotics on the gut microbiota of tilapia using culture-independent methods. Such methods are crucial to understand the mechanisms which underpin and mediate host benefits. © 2010 The Society for Applied Microbiology.

Item Type: Article
Uncontrolled Keywords: fish, health, histology, PCR-DGGE, probiotic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 21 Aug 2017 18:01
Last Modified: 20 Aug 2019 10:15
URI: http://repository.essex.ac.uk/id/eprint/20207

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