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Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers

Teif, VB and Rippe, K (2011) 'Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers.' Physical Biology, 8 (4). 044001-044001. ISSN 1478-3967

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A recent study of transcription regulation in Drosophila embryonic development revealed a complex non-monotonic dependence of gene expression on the distance between binding sites of repressor and activator proteins at the corresponding enhancer cis-regulatory modules (Fakhouri et al 2010 Mol. Syst. Biol. 6 341). The repressor efficiency was high at small separations, low around 30 bp, reached a maximum at 50-60 bp, and decreased at larger distances to the activator binding sites. Here, we propose a straightforward explanation for the distance dependence of repressor activity by considering the effect of the presence of a nucleosome. Using a method that considers partial unwrapping of nucleosomal DNA from the histone octamer core, we calculated the dependence of activator binding on the repressor-activator distance and found a quantitative agreement with the distance dependence reported for the Drosophila enhancer element. In addition, the proposed model offers explanations for other distance-dependent effects at eukaryotic enhancers. © 2011 IOP Publishing Ltd.

Item Type: Article
Uncontrolled Keywords: Nucleosomes; Animals; Drosophila; Drosophila Proteins; Transcription Factors; Repressor Proteins; Enhancer Elements, Genetic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Oct 2017 10:15
Last Modified: 06 Jan 2022 14:49

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