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The application of hybrid pixel detectors for in-house SAXS instrumentation with a view to combined chromatographic operation.

Wright, Gareth SA and Lee, Hyun Chul and Schulze-Briese, Clemens and Grossmann, J Günter and Strange, Richard W and Hasnain, S Samar (2013) 'The application of hybrid pixel detectors for in-house SAXS instrumentation with a view to combined chromatographic operation.' Journal of Synchrotron Radiation, 20 (Pt 2). 383 - 385. ISSN 0909-0495

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Abstract

This study analyses the potential for laboratory-based size-exclusion chromatography (SEC) integrated small-angle X-ray scattering (SAXS) instrumentation to characterize protein complexes. Using a high-brilliance home source in conjunction with a hybrid pixel X-ray detector, the efficacy of SAXS data collection at pertinent protein concentrations and exposure times has been assessed. Scattering data from SOD1 and from the complex of SOD1 with its copper chaperone, using 10 min exposures, provided data quality in the range 0.03 < q < 0.25 Å(-1) that was sufficient to accurately assign radius of gyration, maximum dimension and molecular mass. These data demonstrate that a home source with integrated SEC-SAXS technology is feasible and would enable structural biologists studying systems containing transient protein complexes, or proteins prone to aggregation, to make advanced preparations in-house for more effective use of limited synchrotron beam time.

Item Type: Article
Uncontrolled Keywords: Humans, Superoxide Dismutase, Proteins, Molecular Chaperones, Chromatography, Gel, X-Ray Diffraction, Protein Conformation, Protein Structure, Quaternary, X-Rays, Scattering, Small Angle, Superoxide Dismutase-1
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 13 Apr 2021 13:13
Last Modified: 13 Apr 2021 14:15
URI: http://repository.essex.ac.uk/id/eprint/21176

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