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A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.

Petrini, Edoardo and Baillet, Victoire and Cridge, Jake and Hogan, Cassandra J and Guillaume, Cindy and Ke, Huiling and Brandetti, Elisa and Walker, Simon and Koohy, Hashem and Spivakov, Mikhail and Varga Weisz, Patrick (2015) 'A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.' Journal of Cell Science, 128 (20). 3707 - 3713. ISSN 0021-9533

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Abstract

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

Item Type: Article
Uncontrolled Keywords: Schizosaccharomyces, Phosphates, Myosin Heavy Chains, Schizosaccharomyces pombe Proteins, Signal Transduction, Endocytosis, Gene Expression Regulation, Fungal, Gene Deletion, Actin-Related Protein 2, Transcriptome
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 22 Apr 2021 12:11
Last Modified: 22 Apr 2021 12:11
URI: http://repository.essex.ac.uk/id/eprint/21420

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