Research Repository

Three-dimensional reconstruction of the antennal lobe in Drosophila melanogaster

Laissue, PP and Reiter, C and Hiesinger, PR and Halter, S and Fischbach, KF and Stocker, RF (1999) 'Three-dimensional reconstruction of the antennal lobe in Drosophila melanogaster.' Journal of Comparative Neurology, 405 (4). pp. 543-552. ISSN 0021-9967

Full text not available from this repository.


We present the first three-dimensional map of the antennal lobe of Drosophila melanogaster, based on confocal microscopic analysis of glomeruli stained with the neuropil-specific monoclonal antibody nc82. The analysis of confocal stacks allowed us to identify glomeruli according to the criteria shape, size, position, and intensity of antibody labeling. Forty glomeruli were labeled by nc82, eight of which have not been described before. Three glomeruli previously shown exclusively by backfills were not discernible in nc82 stainings. We distinguish three classes of glomeruli: (1) 'landmark' glomeruli that are constant in all four criteria mentioned above, (2) less well-demarcated glomeruli that deviate in a single criterion, and (3) poorly defined glomeruli that vary in more than one criterion. All class 2 and 3 glomeruli can be identified by comparison with landmark neighbors. To further aid identification, our model assigns glomeruli to five arrays, each of which is defined by a prominent landmark glomerulus. Six glomeruli consist of distinct, but contiguous structural units, termed 'compartments.' Glomerular variability observed occasionally between males and females is in the same range as between individuals of the same sex, suggesting the lack of a significant sexual dimorphism in the glomerular pattern. We compare the new model with a previous map and address its potential for mapping activity and expression patterns. An important goal of this work was to create three- dimensional reference models of the antennal lobe, which are accessible on- line.

Item Type: Article
Uncontrolled Keywords: olfactory system; glomeruli; confocal microscopy; enhancer trap lines; rendering; VRML
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 06 Mar 2018 09:45
Last Modified: 06 Jan 2022 14:51

Actions (login required)

View Item View Item