Research Repository

Three-Dimensional Visualisation and Quantification of Lipids in Microalgae Using Confocal Laser Scanning Microscopy

Chansawang, N and Obara, B and Geider, RJ and Laissue, PP (2016) 'Three-Dimensional Visualisation and Quantification of Lipids in Microalgae Using Confocal Laser Scanning Microscopy.' In: Mcgenity, T and Timmis, K and Nogales, B, (eds.) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks . Springer Verlag, 145 - 161. ISBN 978-3-662-49134-8

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Abstract

Fluorescence microscopy and digital imaging allow the selective visualisation and quantification of cellular components and can convey research findings in an appealing and intuitive way. These techniques are regularly used in biomedical research laboratories, but have less widespread application in marine sciences. We present here an approach to label and volumetrically quantify neutral lipids, chloroplasts, DNA and cell volumes in microalgae. Using fluorescence microscopy techniques on “turn-key” systems commonly available to environmental research labs, imaging facilities or accessible groups in other disciplines ensure that this approach can be widely reproduced.

Item Type: Book Section
Uncontrolled Keywords: Autofluorescence; Chlorophyll; Fluorescence microscopy; Image processing; Lipids; Localisation; Microalgae; Nile red; Quantification; Volumetry
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 05 Mar 2018 10:31
Last Modified: 28 Aug 2019 21:16
URI: http://repository.essex.ac.uk/id/eprint/21630

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