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Identification of leaf promoters for use in transgenic wheat

Alotaibi, SS and Sparks, CA and Parry, MAJ and Simkin, AJ and Raines, CA (2018) 'Identification of leaf promoters for use in transgenic wheat.' Plants, 7 (2). ISSN 2223-7747

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Abstract

Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

Item Type: Article
Uncontrolled Keywords: promoter; wheat; tissue specific; photosynthesis; reporter gene; yield
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Elements
Date Deposited: 11 Jul 2018 14:47
Last Modified: 11 Jul 2018 14:47
URI: http://repository.essex.ac.uk/id/eprint/22647

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