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Unexpected Connections between Humidity and Ion Transport Discovered using a Model to Bridge Guard Cell-to-Leaf Scales

Wang, Yizhou and Hills, Adrian and Vialet-Chabrand, Silvere RM and Papanatsiou, Maria and Griffiths, Howard and Rogers, Simon and Lawson, Tracy and Lew, Virgilio and Blatt, Michael R (2017) 'Unexpected Connections between Humidity and Ion Transport Discovered using a Model to Bridge Guard Cell-to-Leaf Scales.' The Plant Cell, 29 (11). pp. 2921-2939. ISSN 1040-4651

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Stomatal movements depend on the transport and metabolism of osmotic solutes that drive reversible changes in guard cell volume and turgor. These processes are defined by a deep knowledge of the identities of the key transporters and of their biophysical and regulatory properties, and have been modeled successfully with quantitative kinetic detail at the cellular level. Transpiration of the leaf and canopy, by contrast, is described by quasilinear, empirical relations for the inputs of atmospheric humidity, CO2, and light, but without connection to guard cell mechanics. Until now, no framework has been available to bridge this gap and provide an understanding of their connections. Here, we introduce OnGuard2, a quantitative systems platform that utilizes the molecular mechanics of ion transport, metabolism, and signaling of the guard cell to define the water relations and transpiration of the leaf. We show that OnGuard2 faithfully reproduces the kinetics of stomatal conductance in Arabidopsis thaliana and its dependence on vapor pressure difference (VPD) and on water feed to the leaf. OnGuard2 also predicted with VPD unexpected alterations in K+ channel activities and changes in stomatal conductance of the slac1 Cl− channel and ost2 H+-ATPase mutants, which we verified experimentally. OnGuard2 thus bridges the micro-macro divide, offering a powerful tool with which to explore the links between guard cell homeostasis, stomatal dynamics, and foliar transpiration.

Item Type: Article
Uncontrolled Keywords: Arabidopsis; Plant Leaves; Water; Humidity; Signal Transduction; Plant Transpiration; Ion Transport; Kinetics; Mutation; Models, Biological; Plant Stomata; Vapor Pressure
Subjects: Q Science > QK Botany
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 23 Jul 2018 14:51
Last Modified: 18 Aug 2022 13:26

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