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Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography

Ebrahim, Ali and Appleby, Martin V and Axford, Danny and Beale, John and Moreno-Chicano, Tadeo and Sherrell, Darren A and Strange, Richard W and Hough, Michael A and Owen, Robin L (2019) 'Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.' Acta Crystallographica Section D Structural Biology, 75 (2). pp. 151-159. ISSN 2059-7983

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The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were successfully separated to determine two independent structures, and an X-ray-driven change between these polymorphs was followed. This was achieved through the determination of multiple serial structures from microcrystals using a high-throughput high-speed fixed-target approach coupled with robust data processing.

Item Type: Article
Uncontrolled Keywords: radiation damage; fixed-target serial crystallography; metalloproteins; polymorphism; room temperature
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 26 Apr 2019 11:33
Last Modified: 18 Aug 2022 12:30

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