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Introduction to the Isolation and Cultivation of Microbes Involved in the Hydrocarbon Cycle

McGenity, Terry J (2016) 'Introduction to the Isolation and Cultivation of Microbes Involved in the Hydrocarbon Cycle.' In: McGenity, Terry J and Timmis, Kenneth N and Nogales, Balbina, (eds.) Hydrocarbon and Lipid Microbiology Protocols: Isolation and Cultivation. Springer Protocols Handbooks . Springer. ISBN 978-3-662-45179-3

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Our knowledge of the uncultivated microbial majority is driving innovative approaches to cultivation, alongside novel cultivation-independent methods, to better understand microbial ecophysiology, biochemistry, and evolution. Biotechnological applications also provide a major impetus for obtaining microbial cultures, particularly in the field of hydrocarbon and lipid cycling, which address important societal challenges, including: biofuel production, anaerobic digestion, bioplastic synthesis, and mitigating the effects of diverse hydrocarbon pollutants and climate-active gases. This chapter provides a brief overview of cultivation strategies, focussing on those approaches that are most relevant to hydrocarbon cycling. The areas covered include: sample handling, strategies for delivery of carbon and energy sources and terminal electron acceptors, consideration of the physicochemical environment, supply of metabolites and undefined environmental components, assays for microbes, and preservation of cultures.

Item Type: Book Section
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 02 Jan 2019 10:51
Last Modified: 28 Aug 2019 21:16

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