Research Repository

In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones

Fellows, Rachel and Varga Weisz, Patrick (2018) 'In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones.' Bio-protocol, 8 (14). ISSN 2331-8325

[img]
Preview
Text
Bio-protocol2924.pdf - Published Version

Download (2MB) | Preview

Abstract

Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the effect of inhibitors and estimate kinetic constants.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Elements
Date Deposited: 14 Jan 2019 13:15
Last Modified: 14 Jan 2019 13:15
URI: http://repository.essex.ac.uk/id/eprint/23815

Actions (login required)

View Item View Item