Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Abstract
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the effect of inhibitors and estimate kinetic constants.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Acetylation; Butyrylation; Chromatin; Crotonylation; Enzyme; Histone deacetylase; Kinetic |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
| SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
| Depositing User: | Unnamed user with email elements@essex.ac.uk |
| Date Deposited: | 14 Jan 2019 13:15 |
| Last Modified: | 24 May 2022 16:13 |
| URI: | http://repository.essex.ac.uk/id/eprint/23815 |
Available files
Filename: Bio-protocol2924.pdf