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FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM

Wick, U and Kirsch, M and Rauch, A and Chudoba, I and Lausen, B and Efferth, T and Gebhart, E (2005) 'FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM.' Cytogenetic and Genome Research, 111 (1). pp. 34-40. ISSN 1424-8581

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Abstract

So far, the problem of an influence of translocations on the telomeres of the involved chromosomes has not been addressed yet in human cells. Therefore, the telomeres of a karyotypically rather well characterized T-cell acute lymphoblastic leukemia (T-ALL) cell line (CCRF-CEM) with several marker chromosomes were examined using peptide nucleic acid (PNA) telomere FISH probes to compare the telomere length of these markers with that of the chromosome arms of their origin. In addition, chromosome libraries, centromeric probes, and subtelomeric DNA probes were used to further define the marker chromosomes. Two markers could be newly defined and a concise karyotype of the cell line could be obtained by these detailed examinations: 42?47,X,?X,del(5) (q35?),t(5;15)(q14;q13.2),t(8;9)(p11;p24),del(9)(:p13?qter)/inv(9)(pter?p12::q21?p12::q21?qter),+13,+20,+der(22)(p+ [HSR?])[cp]. The relative telomere length of all chromosomes showed considerable interchromosomal, intercellular, and inter-passage variation. However, it could be shown, that in four different passages of the examined cell line the observed differences between relative telomere lengths of the markers and the chromosomes of their origin, with two exceptions (short arms of del/inv9 and der22), were not significant. On the other hand, because of its mentioned variability, telomere length alone is not sufficient to reliably define the derivation of markers.

Item Type: Article
Uncontrolled Keywords: Cell Line, Tumor; Telomere; Humans; Chromosome Aberrations; Genetic Markers; In Situ Hybridization, Fluorescence; Chromosome Mapping; Karyotyping; Leukemia-Lymphoma, Adult T-Cell
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Mathematical Sciences, Department of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 30 May 2012 20:25
Last Modified: 06 Jan 2022 13:24
URI: http://repository.essex.ac.uk/id/eprint/2461

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